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runx 2  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
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    Structured Review

    Cell Signaling Technology Inc runx 2
    Runx 2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/runx+2/pmc13019078-71-4-23
    Average 86 stars, based on 1 article reviews
    runx 2 - by Bioz Stars, 2026-09
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: GIPC2 regulation of the PKM2/SREBP1 signaling axis controls adipogenic differentiation of mesenchymal stem cells
    Article Snippet: .. The membranes were then washed with TBST and incubated overnight at 4 °C with the following primary antibodies, which were diluted as specified by the manufacturers: GIPC2 (Abcam, ab175272, 1:1000), PKM2 (CST, 46687, 1:1000), SREBP1 (CST, 95879, 1:1000), C/EBP-α (Abcam, ab40764, 1:1000), FABP4 (Abcam, ab92501, 1:2500), PPAR-γ (CST, 2443, 1:1000), SOX-9 (Abcam, ab185966, 1:5000), Collagen II (Proteintech, 28459-1-AP, 1:1000), RUNX 2 (CST, 8486, 1:1000), IBSP (Invitrogen, PA5-114915, 1:1000), Phospho-PKM2 (Invitrogen, PA5-37684, 1:1000), Histone H3 (CST, 4499, 1:2000), GAPDH (CST, 5174, 1:1000), Vinculin (CST, 13901, 1:1000) and β-Actin (CST, 4970, 1:1000). .. After incubation with the primary antibodies, the membranes were washed three times with TBST and incubated with the appropriate horseradish peroxidase-conjugated secondary antibodies (absin, 20002, 1:5000) for 2 h at room temperature.

    Article Title: Mechanotransduction-driven macrophage polarization via Integrin-SRC-STAT6 pathway in distraction osteogenesis
    Article Snippet: The proteins were separated through sodium dodecyl sulfate polyacrylamide (SDS-page) gel electrophoresis using a Bio-Rad electrophoresis system, and then the proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane. .. The PVDF membrane with blots was blocked in the tris-buffered saline with 5 %(w/v) skim milk powder at room temperature for 1 h. And then, Incubated the membrane with the primary antibody against the target protein overnight at 4 °C, which included iNOS (1:1000,ab15323, Abcam, Cambridge, UK), Integrin-β (1:1000, #4706, Cell Signaling Technology, Danvers, USA), STAT6 (1:1000, # 5397, Cell Signaling Technology, Danvers, USA), p-STAT6 (1:1000, #56554, Cell Signaling Technology, Danvers, USA), SRC (1:1000, #2109, Cell Signaling Technology, Danvers, USA), p-SRC (1:1000, #6943, Cell Signaling Technology, Danvers, USA), Arg-1 (1:500, PTM-5730, PTMBio, Hangzhou, China), GAPDH (1:5000, GB11002, Servicebio, Wuhan, China), Col-1 (1:1000, ab88147, Abcam, Cambridge, UK), Runx-2 (1:1000, #12556, Cell Signaling Technology, Danvers, USA), OCN (1:1000, AF6300, Beyotime, Shanghai, China). ..

    Membrane:

    Article Title: Mechanotransduction-driven macrophage polarization via Integrin-SRC-STAT6 pathway in distraction osteogenesis
    Article Snippet: The proteins were separated through sodium dodecyl sulfate polyacrylamide (SDS-page) gel electrophoresis using a Bio-Rad electrophoresis system, and then the proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane. .. The PVDF membrane with blots was blocked in the tris-buffered saline with 5 %(w/v) skim milk powder at room temperature for 1 h. And then, Incubated the membrane with the primary antibody against the target protein overnight at 4 °C, which included iNOS (1:1000,ab15323, Abcam, Cambridge, UK), Integrin-β (1:1000, #4706, Cell Signaling Technology, Danvers, USA), STAT6 (1:1000, # 5397, Cell Signaling Technology, Danvers, USA), p-STAT6 (1:1000, #56554, Cell Signaling Technology, Danvers, USA), SRC (1:1000, #2109, Cell Signaling Technology, Danvers, USA), p-SRC (1:1000, #6943, Cell Signaling Technology, Danvers, USA), Arg-1 (1:500, PTM-5730, PTMBio, Hangzhou, China), GAPDH (1:5000, GB11002, Servicebio, Wuhan, China), Col-1 (1:1000, ab88147, Abcam, Cambridge, UK), Runx-2 (1:1000, #12556, Cell Signaling Technology, Danvers, USA), OCN (1:1000, AF6300, Beyotime, Shanghai, China). ..

    Saline:

    Article Title: Mechanotransduction-driven macrophage polarization via Integrin-SRC-STAT6 pathway in distraction osteogenesis
    Article Snippet: The proteins were separated through sodium dodecyl sulfate polyacrylamide (SDS-page) gel electrophoresis using a Bio-Rad electrophoresis system, and then the proteins were transferred onto a polyvinylidene fluoride (PVDF) membrane. .. The PVDF membrane with blots was blocked in the tris-buffered saline with 5 %(w/v) skim milk powder at room temperature for 1 h. And then, Incubated the membrane with the primary antibody against the target protein overnight at 4 °C, which included iNOS (1:1000,ab15323, Abcam, Cambridge, UK), Integrin-β (1:1000, #4706, Cell Signaling Technology, Danvers, USA), STAT6 (1:1000, # 5397, Cell Signaling Technology, Danvers, USA), p-STAT6 (1:1000, #56554, Cell Signaling Technology, Danvers, USA), SRC (1:1000, #2109, Cell Signaling Technology, Danvers, USA), p-SRC (1:1000, #6943, Cell Signaling Technology, Danvers, USA), Arg-1 (1:500, PTM-5730, PTMBio, Hangzhou, China), GAPDH (1:5000, GB11002, Servicebio, Wuhan, China), Col-1 (1:1000, ab88147, Abcam, Cambridge, UK), Runx-2 (1:1000, #12556, Cell Signaling Technology, Danvers, USA), OCN (1:1000, AF6300, Beyotime, Shanghai, China). ..

    other:

    Article Title: Crab shell polypeptides enhance calcium dynamics and osteogenic activity in osteoporosis
    Article Snippet: RUNX-2 , # 12556 , 1:1000 , 1:100 , Cell Signaling Technology.



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